Composite

Part:BBa_K5217035

Designed by: Wanjun Chen   Group: iGEM24_BGI-MammothEdu-East   (2024-09-27)


pTDH3_tLsCl_ADH1t

pTDH3 is a promoter. It is added before the gene tLsCl and followed by the ADH1 terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 970
    Illegal EcoRI site found at 1918
    Illegal PstI site found at 850
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 970
    Illegal EcoRI site found at 1918
    Illegal NheI site found at 1323
    Illegal PstI site found at 850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 970
    Illegal EcoRI site found at 1918
    Illegal BglII site found at 1379
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 970
    Illegal EcoRI site found at 1918
    Illegal PstI site found at 850
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 970
    Illegal EcoRI site found at 1918
    Illegal PstI site found at 850
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1366


[edit]
Categories
Parameters
None